The Londoner - real name Oladeji Daniel Olatunji - had fought twice before, losing both times.
He was beaten in 2018 by Jake Paul – who has since turned pro – and then by TikToker Vinnie Hacker last June.
And this third time was not lucky, because after five rounds, he lost a split decision at the O2 Arena in London.
To his credit, Daisy had clearly improved both his boxing skills and fitness for this venture in the ring.
The bout descended into chaos during round two when a fame-hungry supporter ran into the ring.
It's not clear why she did this other than trying to go viral - but the internet has taken over for all the wrong reasons.
He was embarrassingly thrown out of the ring head first and his actions caused a momentary delay in the fight.
Some guy just ran into the ring in the Deji VS Alex Wassabi fight! pic.twitter.com/k8yIGG2IRh
— trippy (@Martin38764811) March 5, 2022
When it resumed, Deezi barely showed head movement, slamming punches but failing to counter.
Wasabi lacked any real technique but was more relentless than his British rival.
To the dismay of Daisy fans inside Wembley Arena, it was enough on the scorecard to win them a split-decision.
Wasabi, 31, said: "I had no idea how hard it would be. These guys love their people."
Then said to Desi: "Look man, I love you brother. I had already let go of all those things."
Deji, whose brother KSI cheered from ringside, vowed that he would never fight again if he was defeated.
And the now-0-3 former gamer admitted: "I think I think too much when I box. I don't punch enough.
"Well done Alex. I can't dispute that. Thanks to everyone who came out."
Dezi, 25, asked fans if they wanted to see her fight again - which was met with a cheer - before she and Wasabi shook hands over a rematch.
So maybe it could be the fourth time lucky for Deeji.